Analysis Note

ControlRat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.

Application

Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.

Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.

Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeuroregenerative Medicine

Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Immunogen

GAP-43 purified from rat brain

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Format: Purified

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 µg of rat brain lysates.

Specificity

GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein's phosphorylation state.

Storage and Stability

Maintain at 2-8°C in undiluted for up to 6 months after date of receipt.

Target description

43-48 kDa